Tris- EDTA (TE)

 
 

Overview: TE is a basic laboratory buffer. It is commonly used to preserve extracted nucleic acids.

To view a PDF of this protocol: Tris-EDTA Recipe


Materials:

- Tris(hydroxymethyl)aminomethane (Tris)

- Ethylenediaminetetraacetic acid (EDTA)

- Deionized (DI) water

- Hydrochloric Acid (HCl) for pH adjustment


Lab equipment needed:

- flask

- stir bar and stir plate

- scale

- graduated cylinder

- pipet

- pH reader



To make 1L of 1x TE


1.Add 10mL of 1M Tris, 2mL of 0.5M EDTA, and 988mL DI water. Mix with a stir bar until homogenous.



To make 10mL of 1M Tris

-Add 1.211g Tris in 10mL DI water

The math:

(0.01L=10mL)(1M)= 0.01 moles of Tris

(0.01 moles)(formula weight of Tris=121.14g/mol)= 1.211g

-Adjust pH to between 7.5 and 8, if necessary


To make 2mL of 0.5M EDTA:

-Add 0.37g EDTA to 2mL DI water

The math:

(0.002L=2mL)(0.5M)= 0.001 moles of EDTA

(0.001 moles)(formula weight of EDTA= 372.24g/mol)= 0.37g

-Adjust pH to 8